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What mRNA sequence does the following template DNA nucleotide sequence specify?

November 2, 2020

What mRNA sequence does the following template DNA nucleotide sequence specify?

5’TTTTAGCTTTTTGGCATCA3′

Answer: 5’UGAUGCAAAAAGCUAAAA3′

What is the small protein sequence that will result from the mRNA that is made in the question above?

a) phe-stop-leu-phe-gly-ile

b) met-pro-lys-ser

c) lys-ile-glu-lys-pro

d) thr-thr-cal-pre-arg-phe

Answer

Answer c) lys-ile-glu-lys-pro

5’UGAUGCAAAAAGCUAAAA3′ we translate from the 3′ end towards 5′ end

The protein sequence could be lys-ile-glu-lys-pro-Ser

The shorter sequence should then be lys-ile-glu-lys-pro

Explanation

It is first important to note that each codon consists of three nucleotides, usually corresponding to a single amino acid.

The template DNA is first transcribed in that A is substituted with U and G substituted with C to form the corresponding mRNA which is 5’UGAUGCAAAAAGCUAAAA3′

We divide the mRNA into sections of three nucleotides and then find the amino acid for each codon as follows

5’UGA-UGC-AAA-AAG-CUA-AAA3′

We start from the 3′ end

AAA – Lys

AUC – ile

GAA – Glu

AAA -Lys

CGU -Pro

AGU – Ser

The protein sequence is c) lys-ile-glu-lys-pro

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