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What mRNA sequence does the following template DNA nucleotide sequence specify?

What mRNA sequence does the following template DNA nucleotide sequence specify?


        Answer: 5’UGAUGCAAAAAGCUAAAA3′

What is the small protein sequence that will result from the mRNA that is made in the question above?

        a) phe-stop-leu-phe-gly-ile

        b) met-pro-lys-ser

        c) lys-ile-glu-lys-pro

        d) thr-thr-cal-pre-arg-phe


  Answer  c) lys-ile-glu-lys-pro

5’UGAUGCAAAAAGCUAAAA3′ we translate from the 3′ end towards 5′ end

The protein sequence could be  lys-ile-glu-lys-pro-Ser

The shorter sequence should then be lys-ile-glu-lys-pro


It is first important to note that each codon consists of three nucleotides, usually corresponding to a single amino acid.

The template DNA is first transcribed in that A is substituted with U and G substituted with C to form the corresponding mRNA which is 5’UGAUGCAAAAAGCUAAAA3′

We divide the mRNA into sections of three nucleotides and then find the amino acid for each codon as follows


We start from the 3′ end

AAA – Lys

AUC – ile

GAA – Glu

AAA -Lys

CGU -Pro

AGU – Ser

The protein sequence is     c) lys-ile-glu-lys-pro

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